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        "description": "Objective 1. Characterisation of BCG-Induced protective cells. By characterizing the incomplete immunity induced by BCG, we may be able to design improved vaccines which stimulate better or additional long term memory responses. A prerequisite for this is more tractable measurement of long term memory responses in cattle, and understanding BCG protection in mice is an optimal approach to achieve this. One of the approaches being undertaken is RNA-Seq & combined bio-informatic analysis on highly specific T cells from immune mice, isolated by flow cytometric sorting. Additional information: Please note that Defra procurement uses e-tendering, provided by Bravosolutions. In order to access the Bravo system you will need to be registered. Could I therefore request that you register on the Bravo portal for Defra. If you're already registered on the Bravo portal please check that your registration includes Defra, as you have to specify us in order to correctly receive the tender documents. If you have any problems with the registration process, please contact the Bravo helpline on 0800 368 4850 or alternatively email them at help@bravosolution.co.uk. The link you require for new registration/amendments to registrations is - https://defra.bravosolution.co.uk",
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