---
title: "3464/JN - Galvo-Scanning TIRF Microscope System for Single Molecule Imaging"
ocid: "ocds-b5fd17-26251f43-73e5-455d-9819-1785bc2244b2"
canonical_url: "https://d3tenders.com/contract/?ocid=ocds-b5fd17-26251f43-73e5-455d-9819-1785bc2244b2"
markdown_url: "https://d3tenders.com/contract/ocds-b5fd17-26251f43-73e5-455d-9819-1785bc2244b2.md"
json_url: "https://d3tenders.com/contract/ocds-b5fd17-26251f43-73e5-455d-9819-1785bc2244b2.json"
source: "Contracts Finder"
current_stage: "Award"
buyer: "UNIVERSITY OF SHEFFIELD"
published: "2022-10-05"
---

# 3464/JN - Galvo-Scanning TIRF Microscope System for Single Molecule Imaging

Buyer: UNIVERSITY OF SHEFFIELD  
Current stage: Award  
OCID: ocds-b5fd17-26251f43-73e5-455d-9819-1785bc2244b2

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## Summary

The University of Sheffield has awarded a contract for a Galvo-Scanning TIRF Microscope System for Single Molecule Imaging to Cairn Research Ltd. The contract is valued at £294,868.87 GBP. The procurement method was selective, calling off from a framework agreement. The tender period ended on September 13, 2022, and the contract period will start on January 2, 2023, and end on January 3, 2023. The procurement stage is in the award phase, and the contract was recently awarded on September 27, 2022.

This tender presents an opportunity for businesses specialising in the goods category, particularly in microscopes. Small and medium-sized enterprises (SMEs) and voluntary, community, and social enterprises (VCSEs) are considered suitable for this procurement. The University of Sheffield, located in Sheffield, England, initiated this tender to support research in understanding molecular mechanisms related to slow axonal transport. Businesses involved in microscopy equipment and related servicing and maintenance could find this opportunity well-suited for growth and collaboration.

## Notice

This is a contract awarded via mini-competition under Lot 4 (Super Resolution and Enhanced Resolution Microscopes) of the APUC Framework Agreement for: Microscopes and Imaging Equipment including Associated Servicing, Maintenance and Repairs LAB1028 AP The Twelvetrees Lab work on understanding the molecular mechanisms or slow axonal transport so we can develop a deeper understanding of how this process supports neuronal function and longevity. The group uses a combination of biochemistry, cell biology and single molecule methods to probe transport mechanisms, through generous funding from a Sir Henry Dale Fellowship from the Wellcome Trust and the Royal Society. The lab is based in the Sheffield Institute for Translational Neuroscience and collaborates with many groups in the building. Our work studies how microtubule motor proteins interact differentially with a variety of cargo to regulate distinct rates of transport. Many of our experiments are built around fluorescence microscopy assays; we rely on self labelling enzymes (SNAPf, CLIPf and HaloTag) in combination with their fluorescent ligands (e.g. TMR, 647-SiR, JF549 etc) for single molecule imaging, and also use fluorescent protein markers (EGFP, mTurquoise2, mScarlet-I etc). We use two broad classes of assay system: 1. primary neuronal cultures for live cell imaging and; 2. in vitro motile systems reconstituted from purified proteins. Typical microscopy applications of the lab include, but are not limited to: live-cell imaging of primary neurons over time scales from minutes to hours; single-molecule imaging in live cells; single molecule imaging of in vitro reconstituted motile systems; FRET of single molecules in vitro and populations of molecules within cells; photoablation and activation; super resolution microscopy. The University now requires a microscopy system to support the research in this area. In order to image live neurons and in vitro reconstituted systems, the system must be capable of a number of distinct microscopy applications, including but not limited to: single molecule TIRF microscopy, instantaneous FRAP and TIRF, live-cell imaging, FRET, DNA-PAINT, widefield fluorescence microscopy. To facilitate single molecule imaging in demanding conditions, the system must have an automated focus drift compensation system to maintain z-position. To preserve the activity and integrity of live neurons, the system will be housed in a temperature controlled enclosure that can be operated at a range of temperatures, from room temperature up to 37C. The system must be mounted on an anti-vibration table to minimise environmental vibrations interfering with imaging. Computer control of the system must facilitate both sub second time lapse imaging of single molecules as well as automated time lapse imaging of several points in the sample over many hours.

## Key Details

| Field | Value |
| --- | --- |
| Publication source | Contracts Finder |
| Latest notice | https://www.contractsfinder.service.gov.uk/Notice/0315308a-cb55-43c0-97d7-45d1eddbd7a8 |
| Notice type | Award Notice |
| Procurement type | Framework |
| Procurement category | Goods |
| Procurement method | Selective |
| Procurement method details | Call-off from a framework agreement |
| Tender suitability | SME, VCSE |
| Awardee scale | SME |
| All stages | Award |

## Dates

| Field | Value |
| --- | --- |
| Publication date | 5 Oct 2022 |
| Submission deadline | 13 Sep 2022 |
| Future notice date | Not specified |
| Award date | 26 Sep 2022 |
| Contract period | 2 Jan 2023 - 3 Jan 2023 |
| Recurrence | Not specified |

## Values

| Field | Value |
| --- | --- |
| Tender value | £294,868 |
| Lots value | Not specified |
| Awards value | £294,868 |
| Contracts value | Not specified |

## Status

| Field | Value |
| --- | --- |
| Tender status | Complete |
| Lots status | Not specified |
| Awards status | Active |
| Contracts status | Not specified |

## Buyer

| Field | Value |
| --- | --- |
| Main buyer | UNIVERSITY OF SHEFFIELD |
| Locality | SHEFFIELD |
| Post town | Sheffield |
| Postcode | S10 2TN |
| Country | England |
| ITL 1 | TLE Yorkshire and The Humber |
| ITL 2 | TLE3 South Yorkshire |
| ITL 3 | TLE32 Sheffield |
| Local authority | Sheffield |
| Electoral ward | Broomhill and Sharrow Vale |
| Westminster constituency | Sheffield Central |
| Delivery location | TLE Yorkshire and The Humber |

## Supplier

| Field | Value |
| --- | --- |
| Number of suppliers | 1 |
| Supplier names | CAIRN RESEARCH |

## CPV Codes

### Divisions

- 38 - Laboratory, optical and precision equipments (excl. glasses)

### Codes

- 38510000 - Microscopes

## Release History

- 5 Oct 2022 at 12:43 - Award - Award Notice - https://www.contractsfinder.service.gov.uk/Notice/0315308a-cb55-43c0-97d7-45d1eddbd7a8

## Documents

- https://www.contractsfinder.service.gov.uk/Notice/0315308a-cb55-43c0-97d7-45d1eddbd7a8
  5th October 2022 - Awarded contract notice on Contracts Finder

## Provenance

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