--- title: "CEFAS22-26 RFQ for DNA extraction, metabarcoding, and bioinformatics of marine environmental samples for Atlantic Bluefin Tuna (ABFT)" ocid: "ocds-b5fd17-46dc554a-f9c7-4871-98e7-95cb9e274c33" canonical_url: "https://d3tenders.com/contract/?ocid=ocds-b5fd17-46dc554a-f9c7-4871-98e7-95cb9e274c33" markdown_url: "https://d3tenders.com/contract/ocds-b5fd17-46dc554a-f9c7-4871-98e7-95cb9e274c33.md" json_url: "https://d3tenders.com/contract/ocds-b5fd17-46dc554a-f9c7-4871-98e7-95cb9e274c33.json" source: "Contracts Finder" current_stage: "Tender" buyer: "DEFRA NETWORK ETENDERING PORTAL" published: "2022-03-30" --- # CEFAS22-26 RFQ for DNA extraction, metabarcoding, and bioinformatics of marine environmental samples for Atlantic Bluefin Tuna (ABFT) Buyer: DEFRA NETWORK ETENDERING PORTAL Current stage: Tender OCID: ocds-b5fd17-46dc554a-f9c7-4871-98e7-95cb9e274c33 [View canonical contract page](https://d3tenders.com/contract/?ocid=ocds-b5fd17-46dc554a-f9c7-4871-98e7-95cb9e274c33) [Download OCDS JSON](https://d3tenders.com/contract/ocds-b5fd17-46dc554a-f9c7-4871-98e7-95cb9e274c33.json) ## Summary The procurement process titled "CEFAS22-26 RFQ for DNA extraction, metabarcoding, and bioinformatics of marine environmental samples for Atlantic Bluefin Tuna (ABFT)" is facilitated by the Cefas Lowestoft Laboratory, part of the Defra Network eTendering Portal. This tender falls under the analysis services category within the environmental services industry. The procurement is currently in the Tender stage, with a deadline for submissions set for 12th April 2022. The contract value is £30,000 and is expected to commence on 19th April 2022, lasting until 28th June 2022. The tender follows an open procurement method, specifically an open procedure below the threshold. Deliverables must be completed within 10 weeks from the start date. This tender represents a significant opportunity for businesses specialising in environmental analysis, molecular biology, or bioinformatics to expand their portfolios. Companies that excel in DNA extraction, metabarcoding, and data analysis, particularly those with capabilities in conducting qPCR and taxonomic assignment, would be well-positioned to compete. Small and medium-sized enterprises (SMEs) are encouraged to participate, as emphasized by the procurement's suitability criteria. Engaging with this contract could enhance business growth by providing valuable services to a government entity while gaining experience in marine environmental studies. ## Notice Cefas require the successful supplier to provide services for DNA to be extracted from 116 frozen Sterivex cartridges (Millipore; SVGP01050). The cartridges consist of 29 samples in triplicate, and 29 field blanks. Following extraction, Cefas require half the extracted DNA to be sent back to the Cefas laboratory in Lowestoft so we can undertake qPCR (e.g., each of the extracted samples will be halved, one half will be used for metabarcoding, while the other half will be used for qPCR). Cefas require the extracted DNA to undergo metabarcoding using the MiFish 12s marker (Miya et al., 2015) (and MiFish T markers if dual amplicon approach can be achieved). The supplier will also conduct taxonomic assignment on the metabarcoding data using amplicon sequence variants (preferred) or operational taxonomic units and Blast on a Genbank generated database (or similar). Metabarcoding results should be presented to Cefas so that the number of sequences per taxa is available for every sample in a table format. Cefas also require the supplier to provide the raw data for the sequencing run and methodology involved. Cefas will report results in a scientific manuscript, in consequence details on the methodology provided by the supplier will need to be sufficient to pass peer review (proprietary techniques will be protected). Cefas require the supplier to provide weekly updates via email detailing progress on deliverables. Cefas require all deliverables to be completed within 10 weeks of Contract start date. ## Key Details | Field | Value | | --- | --- | | Publication source | Contracts Finder | | Latest notice | https://www.contractsfinder.service.gov.uk/Notice/f184fc0a-ea41-489a-a500-32180353435f | | Notice type | Tender Notice | | Procurement type | Standard | | Procurement category | Services | | Procurement method | Open | | Procurement method details | Open procedure (below threshold) | | Tender suitability | SME | | Awardee scale | Not specified | | All stages | Tender | ## Dates | Field | Value | | --- | --- | | Publication date | 30 Mar 2022 | | Submission deadline | 12 Apr 2022 | | Future notice date | Not specified | | Award date | Not specified | | Contract period | 18 Apr 2022 - 28 Jun 2022 | | Recurrence | Not specified | ## Values | Field | Value | | --- | --- | | Tender value | £30,000 | | Lots value | Not specified | | Awards value | Not specified | | Contracts value | Not specified | ## Status | Field | Value | | --- | --- | | Tender status | Active | | Lots status | Not specified | | Awards status | Not specified | | Contracts status | Not specified | ## Buyer | Field | Value | | --- | --- | | Main buyer | DEFRA NETWORK ETENDERING PORTAL | | Locality | LOWESTOFT | | Post town | Norwich | | Postcode | NR33 0HT | | Country | England | | ITL 1 | TLH East (England) | | ITL 2 | TLH6 Suffolk | | ITL 3 | TLH63 East Suffolk | | Local authority | East Suffolk | | Electoral ward | Kirkley & Pakefield | | Westminster constituency | Lowestoft | | Delivery location | Not specified | ## CPV Codes ### Divisions - 71 - Architectural, construction, engineering and inspection services - 90 - Sewage, refuse, cleaning and environmental services ### Codes - 71620000 - Analysis services - 90700000 - Environmental services ## Release History - 30 Mar 2022 at 12:40 - TenderAmendment - Tender Notice - https://www.contractsfinder.service.gov.uk/Notice/f184fc0a-ea41-489a-a500-32180353435f - 30 Mar 2022 at 12:40 - Tender - Tender Notice - https://www.contractsfinder.service.gov.uk/Notice/f184fc0a-ea41-489a-a500-32180353435f ## Documents - https://www.contractsfinder.service.gov.uk/Notice/f184fc0a-ea41-489a-a500-32180353435f 30th March 2022 - Opportunity notice on Contracts Finder - https://defra.bravosolution.co.uk/go/26627364017FDAD56780 Link to eSourcing Portal ## Provenance This Markdown file is an alternate public rendering of the D3 Tenders contract record. The canonical page is https://d3tenders.com/contract/?ocid=ocds-b5fd17-46dc554a-f9c7-4871-98e7-95cb9e274c33. The underlying structured data is available as OCDS JSON at https://d3tenders.com/contract/ocds-b5fd17-46dc554a-f9c7-4871-98e7-95cb9e274c33.json.