--- title: "CEFAS24-116 Request for Quotation for genotype-by-sequencing approach for estimating familial relations among pollack" ocid: "ocds-b5fd17-7463c582-b587-42dd-8a8d-b10d22341ac9" canonical_url: "https://d3tenders.com/contract/?ocid=ocds-b5fd17-7463c582-b587-42dd-8a8d-b10d22341ac9" markdown_url: "https://d3tenders.com/contract/ocds-b5fd17-7463c582-b587-42dd-8a8d-b10d22341ac9.md" json_url: "https://d3tenders.com/contract/ocds-b5fd17-7463c582-b587-42dd-8a8d-b10d22341ac9.json" source: "Contracts Finder" current_stage: "Tender" buyer: "CEFAS" published: "2024-08-20" --- # CEFAS24-116 Request for Quotation for genotype-by-sequencing approach for estimating familial relations among pollack Buyer: CEFAS Current stage: Tender OCID: ocds-b5fd17-7463c582-b587-42dd-8a8d-b10d22341ac9 [View canonical contract page](https://d3tenders.com/contract/?ocid=ocds-b5fd17-7463c582-b587-42dd-8a8d-b10d22341ac9) [Download OCDS JSON](https://d3tenders.com/contract/ocds-b5fd17-7463c582-b587-42dd-8a8d-b10d22341ac9.json) ## Summary The Centre for Environment, Fisheries and Aquaculture Science (Cefas), located in Lowestoft, United Kingdom, has issued a tender under reference CEFAS24-116. The tender seeks quotations for a genotype-by-sequencing (GBS) approach to estimate familial relationships among pollack populations. This procurement process is in the tender stage, focusing on analysis services within the research laboratory sector. The work involves DNA extraction, quality control, and sequencing for approximately 3000 pollack samples collected from multiple regions in the English Channel, with a deadline for final results by 15th March 2025. The contract is scheduled to commence on 30th September 2024 and will end on 15th March 2025, with all services to be conducted at the supplier's premises within the UK. This tender presents substantial opportunities for businesses specialising in DNA sequencing and genetic analysis, particularly those experienced in handling large-scale marine biology projects. Small and medium-sized enterprises (SMEs) are especially encouraged to compete, as the procurement method adopted is an open procedure below the threshold. By participating, businesses can enhance their portfolio, gain prestige by collaborating with a renowned organisation like Cefas, and potentially secure further projects within the environmental and marine research sectors. Suitable candidates should offer solutions that maximise SNP identification and coverage, essential for investigating kinship levels among pollack. ## Notice Cefas is currently undertaking genetics based Close-Kin Mark-Recapture (CKMR) research on pollack populations. Cefas is seeking a supplier to sequence samples using a Genotype-by-Sequencing (GBS) approach. Samples have been collected from pollack originating from multiple areas across the English Channel with generated Single Nucleotide Polymorphisms (SNPs) being used to identify Parent-Offspring Pairs (POPs) as well as more distant kinship relationships, such as Half Sibling Pairs (HSPs). Cefas is aware that there is a multitude of GBS approaches, some using propriety technology and some not. Cefas are seeking applications which offer a GBS approach that maximises SNP identification and coverage, to allow Cefas to explore kinship levels amongst pollack. To aid with the above project, Cefas require the supplier to carry out DNA extraction of muscle tissues, quality control steps (DNA Quality Control information (i.e. pass/fail based on DNA concentration and DNA quality) supplied to Cefas before genotype-by-sequencing commences) and genotype-by-sequencing of pollack samples. Therefore, suppliers are asked to provide a cost per sample based on the above. As standard, muscle tissue samples are to be provided by Cefas in LVL genetic sampling tubes with analytical grade ethanol and stored in -20oC: https://www.lvl-technologies.com/en/sample-storage/safe-2d-tubes-engl/safe-96-tissue-collection-951. Cefas requires suppliers to provide an estimate of how long it would take to provide results for both 3000 samples and their individual minimum run length to process per sequencing, based on the 'Deliverables' section described below. Deliverables: * DNA extraction of all samples * DNA Quality Control information (i.e. pass/fail based on DNA concentration and DNA quality) supplied to Cefas before genotype-by-sequencing commences. * Genotype-by-sequencing providing SNPS with an average of >30x coverage. o ~3000* tissue samples provided in total (exact amount to be confirmed) * Samples can either be submitted to the supplier as they are received by Cefas OR submitted all at once based on suppliers' flexibility * Sequencing files to be provided in fastq format with a QC Report o Final sample submission date being end of February 2025. o All results, with a final QC report, must be received by 15th of March 2025. *Please note sample number stated above is the current estimated number of samples collected, final sample number may vary Location for services to be undertaken: At the supplier's premises, please specify full details of location within your response. Download the Bidder Pack at https://defra-family.force.com/s/Welcome and search for CEFAS24-116 under opportunities. ## Key Details | Field | Value | | --- | --- | | Publication source | Contracts Finder | | Latest notice | https://www.contractsfinder.service.gov.uk/Notice/9bbd752b-4050-4334-bcab-7f3ab0ecb087 | | Notice type | Tender Notice | | Procurement type | Standard | | Procurement category | Services | | Procurement method | Open | | Procurement method details | Open procedure (below threshold) | | Tender suitability | SME | | Awardee scale | Not specified | | All stages | Tender | ## Dates | Field | Value | | --- | --- | | Publication date | 20 Aug 2024 | | Submission deadline | 10 Sep 2024 | | Future notice date | Not specified | | Award date | Not specified | | Contract period | 29 Sep 2024 - 15 Mar 2025 | | Recurrence | Not specified | ## Values | Field | Value | | --- | --- | | Tender value | £80,000 | | Lots value | Not specified | | Awards value | Not specified | | Contracts value | Not specified | ## Status | Field | Value | | --- | --- | | Tender status | Active | | Lots status | Not specified | | Awards status | Not specified | | Contracts status | Not specified | ## Buyer | Field | Value | | --- | --- | | Main buyer | CEFAS | | Locality | LOWESTOFT | | Post town | Norwich | | Postcode | NR33 0HT | | Country | England | | ITL 1 | TLH East (England) | | ITL 2 | TLH6 Suffolk | | ITL 3 | TLH63 East Suffolk | | Local authority | East Suffolk | | Electoral ward | Kirkley & Pakefield | | Westminster constituency | Lowestoft | | Delivery location | Not specified | ## CPV Codes ### Divisions - 71 - Architectural, construction, engineering and inspection services - 73 - Research and development services and related consultancy services ### Codes - 71620000 - Analysis services - 73111000 - Research laboratory services ## Release History - 20 Aug 2024 at 14:32 - Tender - Tender Notice - https://www.contractsfinder.service.gov.uk/Notice/9bbd752b-4050-4334-bcab-7f3ab0ecb087 - 20 Aug 2024 at 14:18 - Tender - Tender Notice - https://www.contractsfinder.service.gov.uk/Notice/a52f1290-12ec-4777-a5bc-13f36d4759cf - 20 Aug 2024 at 13:42 - Tender - Tender Notice - https://www.contractsfinder.service.gov.uk/Notice/73c7e533-428d-4328-b317-06ae9f2bcc3d ## Documents - https://www.contractsfinder.service.gov.uk/Notice/9bbd752b-4050-4334-bcab-7f3ab0ecb087 20th August 2024 - Opportunity notice on Contracts Finder - https://www.contractsfinder.service.gov.uk/Notice/a52f1290-12ec-4777-a5bc-13f36d4759cf 20th August 2024 - Opportunity notice on Contracts Finder - https://www.contractsfinder.service.gov.uk/Notice/73c7e533-428d-4328-b317-06ae9f2bcc3d 20th August 2024 - Opportunity notice on Contracts Finder ## Notice URLs - https://defra-family.force.com/s/Welcome - https://www.lvl-technologies.com/en/sample-storage/safe-2d-tubes-engl/safe-96-tissue-collection-951 ## Provenance This Markdown file is an alternate public rendering of the D3 Tenders contract record. 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