---
title: "CEFAS22-26 RFQ for DNA extraction, metabarcoding, and bioinformatics of marine environmental samples for Atlantic Bluefin Tuna (ABFT)"
ocid: "ocds-b5fd17-969c0485-1424-47ee-b548-d4a273b74851"
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markdown_url: "https://d3tenders.com/contract/ocds-b5fd17-969c0485-1424-47ee-b548-d4a273b74851.md"
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source: "Contracts Finder"
current_stage: "Award"
buyer: "DEFRA NETWORK ETENDERING PORTAL"
published: "2022-04-20"
---

# CEFAS22-26 RFQ for DNA extraction, metabarcoding, and bioinformatics of marine environmental samples for Atlantic Bluefin Tuna (ABFT)

Buyer: DEFRA NETWORK ETENDERING PORTAL  
Current stage: Award  
OCID: ocds-b5fd17-969c0485-1424-47ee-b548-d4a273b74851

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## Summary

The procurement process, initiated by the Defra Network eTendering Portal, involves the tender titled "CEFAS22-26 RFQ for DNA extraction, metabarcoding, and bioinformatics of marine environmental samples for Atlantic Bluefin Tuna (ABFT)." This contract falls under the analysis services category and is based in Lowestoft, England. The procurement method used is an open procedure, which is typically suitable for services below the threshold. The contract, valued at £20,880, commenced on 20 April 2022 and is set to run until 30 June 2022. The tender period concluded on 12 April 2022, and the process status is marked as complete.

This tender presents significant opportunities for businesses in the life sciences, biotechnology, and environmental analysis sectors, particularly those specialising in DNA extraction and associated bioinformatics services. Small and medium-sized enterprises (SMEs) with expertise in metabarcoding and qPCR methods will find a suitable competitive landscape, especially as the project involves ongoing collaboration and reporting requirements. Companies capable of meeting the scientific and methodological standards necessary for publication would be well-positioned to engage in future tenders of this kind.

## Notice

Cefas has contracted the supplier to provide services for DNA to be extracted from 116 frozen Sterivex cartridges (Millipore; SVGP01050). The cartridges consist of 29 samples in triplicate, and 29 field blanks. Following extraction, Cefas require half the extracted DNA to be sent back to the Cefas laboratory in Lowestoft so we can undertake qPCR (e.g., each of the extracted samples will be halved, one half will be used for metabarcoding, while the other half will be used for qPCR). Cefas require the extracted DNA to undergo metabarcoding using the MiFish 12s marker (Miya et al., 2015) (and MiFish T markers if dual amplicon approach can be achieved). The supplier will also conduct taxonomic assignment on the metabarcoding data using amplicon sequence variants (preferred) or operational taxonomic units and Blast on a Genbank generated database (or similar). Metabarcoding results should be presented to Cefas so that the number of sequences per taxa is available for every sample in a table format. The supplier will also provide the raw data for the sequencing run and methodology involved. Cefas will report results in a scientific manuscript, in consequence details on the methodology provided by the supplier will need to be sufficient to pass peer review (proprietary techniques will be protected). The supplier will provide weekly updates via email detailing progress on deliverables. The supplier will provide all deliverables within 10 weeks of Contract start date.

## Key Details

| Field | Value |
| --- | --- |
| Publication source | Contracts Finder |
| Latest notice | https://www.contractsfinder.service.gov.uk/Notice/88356a7f-868d-4eec-98b8-963940e13971 |
| Notice type | Award Notice |
| Procurement type | Standard |
| Procurement category | Services |
| Procurement method | Open |
| Procurement method details | Open procedure (below threshold) |
| Tender suitability | SME |
| Awardee scale | Large |
| All stages | Award |

## Dates

| Field | Value |
| --- | --- |
| Publication date | 20 Apr 2022 |
| Submission deadline | 12 Apr 2022 |
| Future notice date | Not specified |
| Award date | 19 Apr 2022 |
| Contract period | 19 Apr 2022 - 30 Jun 2022 |
| Recurrence | Not specified |

## Values

| Field | Value |
| --- | --- |
| Tender value | £20,880 |
| Lots value | Not specified |
| Awards value | £20,880 |
| Contracts value | Not specified |

## Status

| Field | Value |
| --- | --- |
| Tender status | Complete |
| Lots status | Not specified |
| Awards status | Active |
| Contracts status | Not specified |

## Buyer

| Field | Value |
| --- | --- |
| Main buyer | DEFRA NETWORK ETENDERING PORTAL |
| Locality | LOWESTOFT |
| Post town | Norwich |
| Postcode | NR33 0HT |
| Country | England |
| ITL 1 | TLH East (England) |
| ITL 2 | TLH6 Suffolk |
| ITL 3 | TLH63 East Suffolk |
| Local authority | East Suffolk |
| Electoral ward | Kirkley & Pakefield |
| Westminster constituency | Lowestoft |
| Delivery location | Not specified |

## Supplier

| Field | Value |
| --- | --- |
| Number of suppliers | 1 |
| Supplier names | APPLIED GENOMICS |

## CPV Codes

### Divisions

- 71 - Architectural, construction, engineering and inspection services

### Codes

- 71620000 - Analysis services

## Release History

- 20 Apr 2022 at 15:11 - AwardUpdate - Award Notice - https://www.contractsfinder.service.gov.uk/Notice/88356a7f-868d-4eec-98b8-963940e13971
- 20 Apr 2022 at 15:11 - Award - Award Notice - https://www.contractsfinder.service.gov.uk/Notice/88356a7f-868d-4eec-98b8-963940e13971

## Documents

- https://www.contractsfinder.service.gov.uk/Notice/88356a7f-868d-4eec-98b8-963940e13971
  20th April 2022 - Awarded contract notice on Contracts Finder
- https://www.contractsfinder.service.gov.uk/Notice/Attachment/019c2899-4f47-44e1-a011-5c21856750ed
  n/a

## Provenance

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