---
title: "OECD 488 transgenic rodent mutation assay (TGRA) using the Big Blue(r) rat"
ocid: "ocds-b5fd17-e9b8c27c-841f-489e-b7d8-3406bcad23b8"
canonical_url: "https://d3tenders.com/contract/?ocid=ocds-b5fd17-e9b8c27c-841f-489e-b7d8-3406bcad23b8"
markdown_url: "https://d3tenders.com/contract/ocds-b5fd17-e9b8c27c-841f-489e-b7d8-3406bcad23b8.md"
json_url: "https://d3tenders.com/contract/ocds-b5fd17-e9b8c27c-841f-489e-b7d8-3406bcad23b8.json"
source: "Contracts Finder"
current_stage: "Award"
buyer: "UNIVERSITY OF SHEFFIELD"
published: "2022-05-27"
---

# OECD 488 transgenic rodent mutation assay (TGRA) using the Big Blue(r) rat

Buyer: UNIVERSITY OF SHEFFIELD  
Current stage: Award  
OCID: ocds-b5fd17-e9b8c27c-841f-489e-b7d8-3406bcad23b8

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## Summary

The procurement process pertains to the University of Sheffield's tender titled "OECD 488 transgenic rodent mutation assay (TGRA) using the Big Blue® rat." This project is classified under the industry category of "Research laboratory services" and is located in Yorkshire and the Humber, United Kingdom. The procurement method is a limited negotiated procedure without prior publication, with a total contract value of £366,300. The tender period concluded on 13th May 2022, and the contract is set to commence on 14th May 2022 and run until 31st May 2023.

This tender presents significant opportunities for businesses focusing on research laboratory services, particularly those experienced in conducting GLP compliant assays and running studies involving transgenic models. Small and medium enterprises (SMEs) with a background in biochemistry, genetics, or laboratory services may find the requirements well-suited to their capabilities. Engaging with this contract could help businesses expand their portfolio and develop strong partnerships within the scientific research community.

## Notice

The conduct of an OECD 488 transgenic rodent mutation assay (TGRA) using the Big Blue(r) rat using cII mutant selection for the University of Sheffield. Studies will be conducted to GLP, and are based on the following preliminary study design: 1. Breeding of target cohort of 46 Big Blue male rats, with delivery of a target of 44 rats to Charles River Laboratories (CRL) facility, Ashland Ohio, USA. 2. Transfer of HPLC analytical method for dose formulation analysis (DFA) and method transfer summary performed by CRL. 3. Method validation, homogeneity, solubility and stability work to enable GLP doseformulation analysis during Big Blue(r) TGRA main study, performed by CRL. 4. 7-day dose-range finding study (DRF), wild type Fischer F344 male and female rats, to determine limit dose of 1000 mg / kg body weight /day or MTD, performed by CRL. 5. Big Blue(r) TGRA rat main study, male only, using vehicle, 3 dose groups and concurrent positive control conducted at CRL. Each group will contain at least 6 male rats, conducted in accordance with OECD 488; daily test item administration by oral gavage for 28 days (positive control will be dosed days 1, 3, 10, 17 & 24 only); termination and schedule necropsy on day 31 with duodenum, liver and bone marrow removed from all animals, flash frozen and stored prior to shipment to Gentronix. 6. Pig-A in vivo mutation analysis will be integrated into the Big Blue(r) TGRA study, with all study animals screened for elevated Pig-A locus mutation in the pre-study acclimatisation phase, and then a blood sample taken from all animals on study on day 31 as a terminal bleed to minimise animal handling procedures and impact onto the Big Blue(r) TGRA study. 7. Dose formulation analysis during conduct of the main study, performed by CRL. 8. Analysis of liver and duodenum (bone marrow will not be analysed initially) from 5 animals per dose group in the in vitro phase of the Big Blue(r) TGRA study as per OECD 488 will be conducted at and performed by Gentronix. DNA will be extracted, packaged into lambda bacteriophage used to infect E. coli strain G1250 to determine phage packaging efficiency and cII mutation detection after plating on agar. Phage packaging titres of at least 125,000 per animal will be determined, to facilitate estimation of mutation frequency. 9. GLP reporting of results. F15: Voluntary ex ante transparency notice (2022/S 000-006619) and F03: Contract award notice (2022/S 000-014913) relate

## Key Details

| Field | Value |
| --- | --- |
| Publication source | Contracts Finder |
| Latest notice | https://www.contractsfinder.service.gov.uk/Notice/8742fb2f-ea5a-4f40-bfc9-ef9633e27715 |
| Notice type | Award Notice |
| Procurement type | Standard |
| Procurement category | Services |
| Procurement method | Limited |
| Procurement method details | Negotiated procedure without prior publication (above threshold) |
| Tender suitability | SME |
| Awardee scale | SME |
| All stages | Award |

## Dates

| Field | Value |
| --- | --- |
| Publication date | 27 May 2022 |
| Submission deadline | 13 May 2022 |
| Future notice date | Not specified |
| Award date | 12 May 2022 |
| Contract period | 13 May 2022 - 31 May 2023 |
| Recurrence | Not specified |

## Values

| Field | Value |
| --- | --- |
| Tender value | £366,300 |
| Lots value | Not specified |
| Awards value | £366,300 |
| Contracts value | Not specified |

## Status

| Field | Value |
| --- | --- |
| Tender status | Complete |
| Lots status | Not specified |
| Awards status | Active |
| Contracts status | Not specified |

## Buyer

| Field | Value |
| --- | --- |
| Main buyer | UNIVERSITY OF SHEFFIELD |
| Locality | SHEFFIELD |
| Post town | Sheffield |
| Postcode | S10 2TN |
| Country | England |
| ITL 1 | TLE Yorkshire and The Humber |
| ITL 2 | TLE3 South Yorkshire |
| ITL 3 | TLE32 Sheffield |
| Local authority | Sheffield |
| Electoral ward | Broomhill and Sharrow Vale |
| Westminster constituency | Sheffield Central |
| Delivery location | TLE Yorkshire and The Humber |

## Supplier

| Field | Value |
| --- | --- |
| Number of suppliers | 1 |
| Supplier names | GENTRONIX |

## CPV Codes

### Divisions

- 73 - Research and development services and related consultancy services

### Codes

- 73111000 - Research laboratory services

## Release History

- 27 May 2022 at 13:03 - Award - Award Notice - https://www.contractsfinder.service.gov.uk/Notice/8742fb2f-ea5a-4f40-bfc9-ef9633e27715

## Documents

- https://www.contractsfinder.service.gov.uk/Notice/8742fb2f-ea5a-4f40-bfc9-ef9633e27715
  27th May 2022 - Awarded contract notice on Contracts Finder

## Provenance

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