--- title: "Knock-out Cell Line Generation" ocid: "ocds-kuma6s-138089" canonical_url: "https://d3tenders.com/contract/?ocid=ocds-kuma6s-138089" markdown_url: "https://d3tenders.com/contract/ocds-kuma6s-138089.md" json_url: "https://d3tenders.com/contract/ocds-kuma6s-138089.json" source: "Sell2Wales" current_stage: "Tender" buyer: "CARDIFF UNIVERSITY" published: "2024-01-17" --- # Knock-out Cell Line Generation Buyer: CARDIFF UNIVERSITY Current stage: Tender OCID: ocds-kuma6s-138089 [View canonical contract page](https://d3tenders.com/contract/?ocid=ocds-kuma6s-138089) [Download OCDS JSON](https://d3tenders.com/contract/ocds-kuma6s-138089.json) ## Summary The procurement process led by Cardiff University is currently at the tender stage for the project titled "Knock-out Cell Line Generation" in the services category. The project involves investigating DNA repair effects on repeat instability through knocking out 10 genes in an immortalised cell line. The tender period ends on 31st January 2024, with the award period ending on 19th February 2024. This tender opportunity by Cardiff University in the research and development services category offers businesses experienced in supplying cell lines within the UK or Europe growth prospects. Companies with proven expertise in cell line provision and DNA repair research are well-suited to compete for this opportunity, contributing to innovative advancements in the field of repeat-induced mutagenesis in Huntington's disease research. ## Notice The title of the wider research project is 'Repeat-induced mutagenesis in Huntington's disease' and the overall aim is to understand how expanded CAG/CTG repeats can cause mutagenesis at the Huntington's disease locus.Huntington's disease (HD) is a progressive neurodegenerative disease characterised by involuntary movements, apathy, and cognitive decline. Expansion of CAG/CTG repeats within the HTT gene cause HD. These repeats are genetically unstable, leading to dramatic changes in repeat size, biased towards expansion over an individual's lifetime. Somatic expansion in affected tissues is thought to modify disease presentation and many genes identified in genome-wide association studies play a role in DNA repair and in repeat instability.Repeat instability, defined as gain or loss of repeat units relies on DNA repair processes although the exact mechanisms are still being elucidated. Although several DNA repair components have been implicated in repeat instability, how they work together and how potential sequence context may influence the process is unclear.Part of this project involves investigating the effects of genes associated with DNA repair on repeat instability. 10 different genes of interest have been identified and to investigate their effect on repeat instability, these genes need to be knocked-out in our custom HEK293 cell line.The requirement is for separate knock-out of 10 genes in an immortalised cell line, GFP(CAG)101, which is HEK293-derived. Vincent Dion et al will provide the original cell line as starting material. This material will be mycoplasma tested before being sent. To express interest, please use the following link and join the Tender Project via the tendering portal.https://in-tendhost.co.uk/cardiffuniversity/aspx/Home(WA Ref:138089) ## Key Details | Field | Value | | --- | --- | | Publication source | Sell2Wales | | Latest notice | https://www.sell2wales.gov.wales/search/search_switch.aspx?ID=138089 | | Notice type | S2W Notice - Website Invitation to Tender Notice | | Procurement type | Standard | | Procurement category | Services | | Procurement method | Open | | Procurement method details | Not specified | | Tender suitability | Not specified | | Awardee scale | Not specified | | All stages | Tender | ## Dates | Field | Value | | --- | --- | | Publication date | 17 Jan 2024 | | Submission deadline | 31 Jan 2024 | | Future notice date | Not specified | | Award date | Not specified | | Contract period | Not specified | | Recurrence | Not specified | ## Values | Field | Value | | --- | --- | | Tender value | Not specified | | Lots value | Not specified | | Awards value | Not specified | | Contracts value | Not specified | ## Status | Field | Value | | --- | --- | | Tender status | Not specified | | Lots status | Not specified | | Awards status | Not specified | | Contracts status | Not specified | ## Buyer | Field | Value | | --- | --- | | Main buyer | CARDIFF UNIVERSITY | | Locality | CARDIFF | | Post town | Cardiff | | Postcode | CF24 0DE | | Country | Wales | | ITL 1 | TLL Wales | | ITL 2 | TLL5 South East Wales | | ITL 3 | TLL52 Cardiff and Vale of Glamorgan | | Local authority | Cardiff | | Electoral ward | Adamsdown | | Westminster constituency | Cardiff East | | Delivery location | Not specified | ## CPV Codes ### Divisions - 73 - Research and development services and related consultancy services ### Codes - 73000000 - Research and development services and related consultancy services - 73100000 - Research and experimental development services - 73110000 - Research services - 73111000 - Research laboratory services - 73200000 - Research and development consultancy services ## Release History - 17 Jan 2024 at 09:28 - Tender - S2W Notice - Website Invitation to Tender Notice - https://www.sell2wales.gov.wales/search/search_switch.aspx?ID=138089 ## Notice URLs - http://www.cardiff.ac.uk/business/why-work-with-us/for-suppliers - https://in-tendhost.co.uk/cardiffuniversity/aspx/Home - https://in-tendhost.co.uk/cardiffuniversity/aspx/Home(WA ## Provenance This Markdown file is an alternate public rendering of the D3 Tenders contract record. The canonical page is https://d3tenders.com/contract/?ocid=ocds-kuma6s-138089. The underlying structured data is available as OCDS JSON at https://d3tenders.com/contract/ocds-kuma6s-138089.json.