Tender

CEFAS24-126 RFQ for Genotype-by-sequencing approach of Colossal Squid

CEFAS

This public procurement record has 2 releases in its history.

Tender

12 Sep 2024 at 11:08

Tender Notice
Tender

12 Sep 2024 at 11:05

Tender Notice
Summary of the contracting process

Cefas (Centre for Environment, Fisheries and Aquaculture Science) is seeking a supplier to undertake a genotype-by-sequencing (GBS) approach for colossal squid samples, as part of a population genetics assessment. This tender falls under the "Analysis services" category and encompasses multiple global locations, including British Oversea Territories and the United Kingdom. The procurement process is at the tender stage, with the bidding period ending on 30th September 2024, and the contract period from 28th October 2024 to 28th February 2025. Key deliverables include DNA quality control reports, GBS data with high sequencing coverage, and sequencing files in compressed fastq format, with all results required by 1st December 2024. The estimated contract value ranges from GBP 20,000 to GBP 40,000.

This tender presents a significant opportunity for businesses specialising in genetic sequencing and DNA analysis to expand their portfolio, particularly those with capabilities in handling rare biological samples and achieving high coverage sequencing. Small and Medium Enterprises (SMEs) with expertise in proprietary or non-proprietary GBS technologies, as well as robust DNA extraction methods, would be well-suited to compete. Success in securing this contract could enhance the reputation and visibility of the winning vendor in the scientific community and international research collaborations, providing a gateway to future public and private sector projects.

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Notice Information

Notice Title

CEFAS24-126 RFQ for Genotype-by-sequencing approach of Colossal Squid

Notice Description

Cefas is currently undertaking a population genetics assessment on colossal squid. Cefas is seeking a supplier to sequence rare samples using a genotype-by-sequencing (GBS) approach. Tissue samples (mainly tentacles) have been collected from various regions across the globe. The samples themselves are in two types of preservative, depending on the institutes which collected the samples; ethanol and formaldehyde. Cefas is aware that there is a multitude of genotype-by-sequencing approaches, some using propriety technology and some not. We are seeking applications which offer a GBS approach that maximises SNP identification and coverage to allow Cefas explore population structure. Additionally, suppliers must have a comprehensive DNA extraction step to compensate for varying levels of sample preservation. Due to the scarcity of these animals, we have 33 samples but we expect samples to be run in duplicate at an average coverage of x30 depth for each duplicated sample. Requirement: To aid with the above project, Cefas require DNA extraction of squid tissues in ethanol (31) and formaldehyde (2). Assessment of DNA quantity and quality (see 'Deliverables' sections) will need to be completed and discussed with Cefas before genotype-by-sequencing of squid samples begins. Cefas are seeking the highest sequencing coverage possible to compensate for minimum samples sizes. Therefore, suppliers are asked to provide a cost per individual sample (n=66, in total). The colossal squid currently has no reference genome. For the benefit of potential suppliers considering which restriction enzymes to use, we supply the following genome information of two closely related species; Estimate genome size = 3.2 Gb (2.74-3.67 Gb) (based on two species, Eupyrmna scolopes and Doryteuthis pleii) GoaT (genomehubs.org). Due to the rarity of samples, we will require the supplier to duplicate extraction of DNA, testing of DNA quality/quantity and genotype-by-sequencing per sample. All samples have been obtained and are ready to be sent as soon as the Contract is finalised. Deliverables: * DNA Quality Control report (i.e. pass/fail based on DNA concentration and DNA quality) supplied to Cefas before genotype-by-sequencing commences. * Genotype-by-sequencing providing SNPS with an average of 30x depth. * Quality control report of genotype-by-sequencing to ensure desired coverage. * Sequencing files to be provided in compressed fastq format. * All results must be received by 1st December 2024. ; Download the Bidder Pack at https://defra-family.force.com/s/Welcome and search for CEFAS24-126 under opportunities.

Notice Details

Publication & Lifecycle

Open Contracting ID
ocds-b5fd17-2dfc328e-f804-47ac-a8aa-a11d5663bb91
Publication Source
Contracts Finder
Latest Notice
https://www.contractsfinder.service.gov.uk/Notice/c0251beb-8269-4ed2-88b6-c446150a5acc
Current Stage
Tender
All Stages
Tender

Procurement Classification

Notice Type
Tender Notice
Procurement Type
Standard
Procurement Category
Services
Procurement Method
Open
Procurement Method Details
Open procedure (below threshold)
Tender Suitability
SME
Awardee Scale
Not specified

Common Procurement Vocabulary (CPV)

CPV Divisions

71 - Architectural, construction, engineering and inspection services

CPV Codes

71620000 - Analysis services

Notice Value(s)

Tender Value
£30,000 Under £100K
Lots Value
Not specified
Awards Value
Not specified
Contracts Value
Not specified

Notice Dates

Publication Date
12 Sep 20241 years ago
Submission Deadline
30 Sep 2024Expired
Future Notice Date
Not specified
Award Date
Not specified
Contract Period
28 Oct 2024 - 28 Feb 2025 1-6 months
Recurrence
Not specified

Notice Status

Tender Status
Active
Lots Status
Not Specified
Awards Status
Not Specified
Contracts Status
Not Specified

Buyer & Supplier

Contracting Authority (Buyer)

Main Buyer
CEFAS
Contact Name
Available with D3 Tenders Premium →
Contact Email
Available with D3 Tenders Premium →
Contact Phone
Available with D3 Tenders Premium →

Buyer Location

Locality
LOWESTOFT
Postcode
NR33 0HT
Post Town
Norwich
Country
England
Major Region (ITL 1)
TLH East (England)
Basic Region (ITL 2)
TLH6 Suffolk
Small Region (ITL 3)
TLH63 East Suffolk
Delivery Location
Not specified
Local Authority
East Suffolk
Electoral Ward
Kirkley & Pakefield
Westminster Constituency
Lowestoft

Further Information

Notice Documents

Open Contracting Data Standard (OCDS)

View full OCDS Record for this contracting process

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The Open Contracting Data Standard (OCDS) is a framework designed to increase transparency and access to public procurement data in the public sector. It is widely used by governments and organisations worldwide to report on procurement processes and contracts. 

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