Award

GB-London: RBGKEW1276 - Whole Genome Sequencing for cacao DNA from herbarium specimens

BIP SOLUTIONS LIMITED

This public procurement record has 1 release in its history.

Award

22 Mar 2024 at 12:36

Summary of the contracting process

The Royal Botanic Gardens, Kew (RBG Kew) has awarded a contract for a high-throughput sequencing service for cacao DNA from historical herbarium collections. This procurement falls under the "Research laboratory services" category and was classified as an open procedure below threshold. The contract value is £17,920 GBP, with a tender deadline of February 16, 2024. The contract period is from March 4, 2024, to September 3, 2025. The buying organisation, BIP SOLUTIONS LIMITED, located in London, was the contracting authority for this tender.

This contract presents an opportunity for small or medium enterprises (SMEs) with experience in high-throughput sequencing from aged herbarium samples. Businesses well-suited to compete are those with expertise in CTAB-extraction methods, library preparation using Nextera XT Library Kit, and paired-end whole genomic sequencing. Being able to handle heavily fragmented DNA and ensure data quality post-sequencing are crucial capabilities. The successful supplier, RBG KEW, will support RBG Kew's project on studying the global geographical history of Theobroma cacao.

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Notice Title

GB-London: RBGKEW1276 - Whole Genome Sequencing for cacao DNA from herbarium specimens

Notice Description

The Royal Botanic Gardens, Kew (RBG Kew) intends to appoint a supplier for a high-throughput sequencing service from ancient-DNA extractions sourced from historical herbarium collections of cacao specimens (Theobroma cacao, Malvaceae) and wild relative species (Theobroma/Herrania spp.). Utilizing a CTAB-extraction method, the extracted samples will be sent in batches. The supplier must conduct pre-quality control (QC) tests (including RNase treatment and DNA purification, if necessary) on these total-genome DNA extractions, ensuring acceptable yields and concentrations. Tasks also include library preparation using the Nextera XT Library Kit and performing paired-end whole genomic sequencing (WGS) using the Illumina NovaSeq 6000 (or an equally powerful sequencing platform) to seek obtaining ideally ~12 Gb per sample, depending on the conditions of the sample. This process aims to cover 120-140 samples, targeting a coverage of 15-30x each, given the known genome size of Theobroma species at ~450-490 Mbp. The supplier should be acquainted with high-throughput sequencing from aged herbarium samples, due to the historical nature of these collections (preserved over 50-100 years ago). They are expected to have previous experience in the production of satisfactory data under these conditions and will need to state this in the application. Each sample sent will contain 25-50 uL of DNA, predominantly at concentrations of 10 ng/uL (some with less, but never less than 0.5 ng/uL of total DNA). It is expected that many samples will have heavily fragmented DNA, and the supplier should be able to present alternatives to deal with these. Following sequencing, the supplier should conduct post-QC to verify data quality before delivery. Data transfer methods such as USB, HDD, or preferably Cloud transfer are viable options. The genomic data obtained will support an ongoing project at RBG Kew. This project aims to comprehensively study the geographical history, and other population genomic studies of Theobroma cacao at a global scale, utilising molecular data obtained from historical herbarium collections combined with data obtained from other sources. Additional information: The contracting authority considers that this contract may be suitable for economic operators that are small or medium enterprises (SMEs). However, any selection of tenderers will be based solely on the criteria set out for the procurement.

Publication & Lifecycle

Open Contracting ID
ocds-b5fd17-da614d87-ee1c-4215-bcc3-c037b1375741
Publication Source
Contracts Finder
Latest Notice
https://www.contractsfinder.service.gov.uk/Notice/8b9908eb-cc22-41d9-a2d6-9e47aa12554f
Current Stage
Award
All Stages
Award

Procurement Classification

Notice Type
Award Notice
Procurement Type
Standard
Procurement Category
Services
Procurement Method
Open
Procurement Method Details
Open procedure (below threshold)
Tender Suitability
SME
Awardee Scale
Large

Common Procurement Vocabulary (CPV)

CPV Divisions

73 - Research and development services and related consultancy services


CPV Codes

73111000 - Research laboratory services

Notice Value(s)

Tender Value
£17,920 Under £100K
Lots Value
Not specified
Awards Value
£17,920 Under £100K
Contracts Value
Not specified

Notice Dates

Publication Date
22 Mar 20241 years ago
Submission Deadline
16 Feb 2024Expired
Future Notice Date
Not specified
Award Date
16 Feb 20242 years ago
Contract Period
4 Mar 2024 - 3 Sep 2025 1-2 years
Recurrence
Not specified

Notice Status

Tender Status
Complete
Lots Status
Not Specified
Awards Status
Active
Contracts Status
Not Specified

Contracting Authority (Buyer)

Main Buyer
BIP SOLUTIONS LIMITED
Contact Name
Available with D3 Tenders Premium →
Contact Email
Available with D3 Tenders Premium →
Contact Phone
Available with D3 Tenders Premium →

Buyer Location

Locality
LONDON
Postcode
TW9 3AB
Post Town
Twickenham
Country
England

Major Region (ITL 1)
TLI London
Basic Region (ITL 2)
TLI7 Outer London - West and North West
Small Region (ITL 3)
TLI75 Hounslow and Richmond upon Thames
Delivery Location
TLI London

Local Authority
Richmond upon Thames
Electoral Ward
Kew
Westminster Constituency
Richmond Park

Supplier Information

Number of Suppliers
1
Supplier Name

RBG KEW

Further Information

Notice Documents

Open Contracting Data Standard (OCDS)

View full OCDS Record for this contracting process

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The Open Contracting Data Standard (OCDS) is a framework designed to increase transparency and access to public procurement data in the public sector. It is widely used by governments and organisations worldwide to report on procurement processes and contracts.

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