Provision of Real Time PCR System and Accessories

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            "description": "Real-time Polymerase Chain Reaction (PCR) equipment"
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        "description": "Provision of Real Time PCR Systems and accesories to the MRC Unit The Gambia at LSHTM. FluidIGM Biomark HD System FluidIGM Juno System FluidIGM C1",
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        "procurementMethodRationale": "The MRC Unit the Gambia at LSHTM believes that the items required are unique and proprietary to the supplier with no other equivalent items available on the marketplace. There are also no resellers within the UK or the Gambia. The BioMark HD System is a unique research and development tool based on proprietary integrated fluidic circuit (IFC) technology that is available solely from Fluidigm. The BioMark HD System is a fully integrated system enabling analysis of gene expression, genotyping, mutant detection, and absolute quantification of nucleic-acid sequences utilizing Digital Array(tm) and/or Dynamic Array(tm) IFC technology. The system is solely produced by Fluidigm and the key components are patented and designed to operate as an integrated system. No third-party systems or components are licensed for use on the BioMark HD System or Digital Array and Dynamic Array IFCs. Data generated by the BioMark HD System from experiments run on IFCs can be exported into a variety of common formats for processing in external third party or proprietary software (which include file export mechanisms supporting the common formats). The Juno(tm) system is available only from Fluidigm that combines integrated fluidic circuit (IFC) control and thermal cycling functions in a single instrument. The key components are patented and designed to operate as an integrated system. It includes the Fluidigm proprietary multilayer soft lithography (MSL(r)) process and silicone Nanoflex(tm) valve technology, which enable high-quality and consistent IFC production and are all unique to Fluidigm. Fluidigm utilizes proprietary design and production processes for all genotyping, gene expression, digital PCR and NGS library preparation IFCs. Each IFC enables researchers to prepare and automate nanoliter-volume reactions with Juno. C1(tm) is a unique and proprietary microfluidics system designed to isolate and prepare individual cells for genomic analysis applications. C1 is a fully integrated system that enables capture, staining for cell viability, imaging of cells for viability and phenotype, cell lysis and template preparation from up to 800 single cells, all on a single platform. The platform is comprised of the C1 system, which includes controller software for IFC loading, priming, and processing; the C1 IFC for cell capture and preparation; and C1 reagent kits containing reagents for cell suspension, lysis and harvest. The system is solely produced by Fluidigm, and the key components are patented and designed to operate as an integrated system. The C1 IFC is a proprietary integrated fluidic circuit that captures and prepares up to 800 individual cells in parallel and is the only IFC compatible with the C1 system. The IFC is based on Fluidigm multilayer soft lithography (MSL(r)) technology, a patented method that enables automatic and precise mixing of reagents into cell-independent, fixed, partitioned nanoliter reaction chambers, preventing sample mix-up or cross contamination. The C1 IFC requires as few as 200 cells for starting material, reduces reagent usage 200-fold and reagent costs by eight-fold, depending on the genomic application."
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